Radioenzymatic assay for catecholamines

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Improved radioenzymatic assay for spectinomycin.

The sensitivity and reproducibility of the radioenzymatic adenylylating assay for spectinomycin were improved by modifications of the assay procedure and by partial purification of the enzyme. The presence of Bacillus subtilis ribosomes or S-150 fraction in the enzymatic reaction mixture interfered with the ability to measure spectinomycin by this method.

متن کامل

Simplified radioenzymatic assay for chloramphenicol.

A simplified radioenzymatic assay for chloramphenicol was developed by eliminating the need for cumbersome extraction procedures. After the acetylation of chloramphenicol with [(14)C]acetyl coenzyme A in the presence of chloramphenicol acetyltransferase, the reaction mixture was added to a toluene-based scintillation fluid. Since (14)C-acetylated chloramphenicol is more soluble than [(14)C]acet...

متن کامل

Radioenzymatic assay of catecholamines in plasma after a preliminary solvent extraction compared with an analogous liquid-chromatographic method.

Catechol-borate complexation and ion-pair formation combined with organic solvent extraction as preliminaries to radioenzymatic assay (REA) with catechol-O-methyltransferase-catalyzed methylation allows the quantification of catecholamines in plasma samples of any volume. We attained improved detection limits for 1-mL samples: 9 ng/L for norepinephrine (NE), 8 ng/L for epinephrine (E), and 11 n...

متن کامل

Carnitine: determination of total carnitine using a radioenzymatic assay.

Acetylcarnitine and acylcarnitine are hydrolyzed by alkaline hydrolysis. The total carnitine is measured by incubation of the sample with 14CacetylCoA and carnitine acetyltransferase. The radioactive acetylcarnitine is separated from the unreacted radioactive acetylCoA using a Dowex 2 x 8 anion exchange column. The radioactive acetylcarnitine fraction is quantitated by counting in a liquid scin...

متن کامل

Radioimmunoassay and radioenzymatic assay of a new aminoglycoside antibiotic, netilmicin.

A radioimmunoassay and a radioenzymatic assay for netilmicin, a new aminoglycoside, were developed in our laboratories to assist in the study of the pharmacology of the drug and to establish values for use in its monitoring. The assays are sensitive, precise, and rapid, giving results that correlate (r = 0.90) with each other and with those of a microbiological assay in which Klebsiella pneumon...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Folia Pharmacologica Japonica

سال: 1978

ISSN: 0015-5691,1347-8397

DOI: 10.1254/fpj.74.885